Annona muricata L., belongs to the family of Annonaceae and has been used for centuries by medicine men in South America to treat a number of ailments. The present investigation was carried out to determine the effect of 2,4-D on Callus Induction and Multiplication of Annona muricata. Callus cultures were initiated from the leaf of the medicinal plant Annona muricata, cultured on Murashige and Skoog (MS) medium supplemented with 30 g/L sucrose and various concentrations (1, 2,3,4and 5 mg/l) of 2,4-dichlorophenoxyacetic acid (2,4-D). Explants treated with various concentration of the same hormone formed calli with different morphologies. In the induction studies, 2,4-D 5 mg/lwas the most efficient formulation for callus formation. The callus induction response was for2,4-D 5mg/l treatment was 84%. In 2,4-D 5mg/l treatment callus was formed between 7-9 days .In 2,4-d3mg/l the callus induction frequency was 89% but the number of days taken for callus induction was 17-22.The least CIF response was in 2,4-D at 4mg/l The CIF response was 35%Calli were successfully Multiplied in 2,4-D 5mg/l treatment with percentage response of 81%. Addition of 2ppm polyvinylpyrrolidone (PVP) aided in overcoming the browning effects by absorbing the phenoliccompounds in the medium to some extent but not so efficiently growth of callus was not affected by browning .